| Aid Station | Cume Miles | AS Delta Miles | Relay Leg Miles | Opening Time | Cutoff Time |
|---|
| Start: Route 28 (map) | 0.0 | | | 6:00 AM | |
| AS1: Zion Road (map)
| 7.9 | 7.9 | | 7:15 AM | |
| AS2/CP: North Freedom (map) | 12.0 | 4.1 | 12.0 | 7:45 AM | 10:00 AM |
| AS3: Mount Tabor (map) | 15.7 | 3.7 | | 8:00 AM | |
| AS4: Route 839 (map) | 18.1 | 2.4 | | 8:15 AM | |
| AS5/CP: Eddyville (map) | 22.0 | 3.9 | 10.0 | 8:45 AM | 12:45 PM |
| AS6: Crossroads Road (map) | 25.4 | 3.4 | | 9:15 AM | |
| AS7/CP: Milton (map) | 29.0 | 3.6 | 7.0 | 9:30 AM | 2:45 PM |
| AS8: Dayton-Smicksburg Road (map) | 34.1 | 5.1 | | 10:15 AM | |
| AS9/CP: Sinktown Road (map) | 39.8 | 5.7 | 10.8 | 10:45 AM | 5:45 PM |
| AS10: Plumville (map) | 42.3 | 2.5 | | 11:00 AM | |
| AS11: Wells Road (map) | 45.7 | 3.4 | | 11:30 AM | |
| Finish: Smicksburg farm (map) | 50.1 | 4.4 | 10.3 | 12:00 AM | 8:30 PM |
